Shift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction
نویسندگان
چکیده
منابع مشابه
Shift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction: Review of Upcoming Techniques
Standard isolation of adipose stromal vascular fraction (SVF) requires the use of collagenase and is considered more than "minimally manipulated" by current good manufacturing practice requirements. Alternatively, nonenzymatic isolation methods have surfaced using physical forces to separate cells from the adipose matrix. The purpose of this study was to review the literature on the use of mech...
متن کاملAbstract: Mechanical Isolation of Adipose-Derived Stromal Vascular Fraction: Is It Becoming a Growing Trend?
MaterialS and MethodS: Adults undergoing liposuction on bilateral body areas were included (n=12). Under sedation, liposuction on one side was conducted with standard tumescent (1L of LR with 30ml of 1% lidocaine and 1 mcg/ml epinephrine). Tumescent without lidocaine was infiltrated on the contralateral side. Five milliliter lipoaspirate samples were processed for isolation of the stromal vascu...
متن کاملAdipose derived stromal vascular fraction improves early tendon healing: an experimental study in rabbits
Tendon never restores the complete biological and mechanical properties after healing. Bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. Stromal vascular fraction (SVF), derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differ...
متن کاملMechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue
Clinical use of adipose-derived stem cells (ASCs) for a variety of indications is rapidly expanding in medicine. Most commonly, ASCs are isolated at the point of care from lipoaspirate tissue as the stromal vascular fraction (SVF). The cells are immediately administered to the patient as an injection or used to enrich fat grafts. Isolation of ASCs from adipose tissue is a relatively simple proc...
متن کاملSuccessful management of an equine carpal chip fracture by intra-articularly injected adipose-derived stromal vascular fraction after arthroscopic removal
Carpal chip fractures are common causes of lameness in racehorses. Due to disadvantages in surgical management, adjuvant treatment modalities are usually necessary. Adipose-derived stem cells (ADSCs) have the potential to differentiate into other cell types including bone and cartilage cells. Adipose-derived stromal vascular fraction (SVF) is produced during ADSCs isolation from adipose tissue....
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ژورنال
عنوان ژورنال: Plastic and Reconstructive Surgery - Global Open
سال: 2016
ISSN: 2169-7574
DOI: 10.1097/gox.0000000000001017